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Native Agarose Gel Electrophoresis of Multiprotein Complexes

Cold Spring Harbor Protocolshttps://cshprotocols.cshlp.org/content/2011/7/pdb.prot4558.abstractNative Agarose Gel Electrophoresis of Multiprotein ComplexesElectrophoresis of proteins and protein–protein complexes in native agarose gels using a horizontal gel apparatus is described here. The procedure is simple to set up, takes a short time to run, and avoids the use of toxic components. The gel is run in a submerged horizontal platform, with the wells positioned in the center of the gel.